Appendix D
About
1
WGS, mRNA-seq, and ChIP-seq
1.1
WGS
1.2
mRNA-seq
1.3
ChIP-seq
2
Transcriptomic Analyses
2.1
Differential gene expression analysis
2.2
WGCNA
3
Allele-Specific Transcriptomic Analyses
3.1
SNPs and transcripts
3.2
POE gene lists
3.3
192hpf WL & QL - allelic transcriptomics
3.3.1
Block 1
3.3.2
Block 2
3.4
24hpf eggs - allelic transcriptomics
3.4.1
Block 3
3.4.2
Block 4
4
ChIP-seq Analysis
4.1
Differential enrichment analysis
4.1.1
H3K27me3
4.1.2
H3K27ac
4.1.3
H3K4me3
5
Allele-Specific ChIP-seq Analyses
5.1
SNPs and peaks
5.2
POE peak lists
5.2.1
H3K27me3
5.2.2
H3K27ac
5.2.3
H3K4me3
5.3
H3K27me3
5.3.1
Gene bodies
5.3.2
Promoters
5.4
H3K27ac
5.4.1
Gene bodies
5.4.2
Promoters
5.5
H3K4me3
5.5.1
Gene bodies
5.5.2
Promoters
6
HPTM-transcription dynamics
6.1
H3K27me3
6.1.1
Gene bodies
6.1.2
Promoters
6.2
H3K27ac
6.2.1
Gene bodies
6.2.2
Promoters
6.3
H3K4me3
6.3.1
Gene bodies
6.3.2
Promoters
7
POE HPTM enrichment-POE transcription dynamics
7.1
Block 1
7.1.1
H3K27me3
7.1.2
H3K27ac
7.1.3
H3K4me3
7.2
Block 2
7.2.1
H3K27me3
7.2.2
H3K27ac
7.2.3
H3K4me3
7.3
Logistic regressions
7.3.1
H3K27me3
7.3.2
H3K27ac
7.3.3
H3K4me3
8
GO enrichment analyses
8.1
DEGs & POE genes
8.2
WGCNA modules
8.3
HPTM differentially enriched regions
9
POE gene feature assessment
9.1
Spatial clustering
9.2
Intron length and recombination rates
Recombination rate
% intron
Published with bookdown
Appendix D: Parent-of-origin variation in transcription and chromatin regulation underly reproductive caste determination
Appendix D-1
WGS, mRNA-seq, and ChIP-seq
1.1
WGS
Blocks 1&2: descriptive statistics for NGS reads
Blocks 3&4: descriptive statistics for NGS reads
1.2
mRNA-seq
192hpf larvae: descriptive statistics for NGS reads
24hpf eggs: descriptive statistics for NGS reads
1.3
ChIP-seq
192hpf larvae: descriptive statistics for NGS reads
Example WL sheared chromatin sample.
Example QL sheared chromatin sample.
Example cross-correlation plot of ChIP-seq library after alignment and filtering.